Characteristics of Blood Samples From Normal Donors and Sickle Cell Anemia Patients

Recent findings that hemoglobin S gelation was greatly increased only between blood and sickling are pH-dependent and also pH 7.4 and 7.2 (cell pH 7.2 and 7.0. a shift influence oxygen affinity suggested that that strongly affects gelation). with L log the red cells containing this hemoglobin p50/ L pH -0.92 to -0.99 (normal variant might show an abnormal Bohr 0.42 to 0.46). Thus a drop in SS blood effect. We therefore studied the effects of pH below 7.4 in tissue capillaries yields pH variation on the in vitro oxygen affinity twice the normal decrease in oxygen affinof whole blood from persons with sickle ity and a large release of oxygen from red cell anemia (SS) and normal donors (at cells, whose risk of sickling is high. Even 3TC and constant carbon dioxide tension mild transient acidosis would seem hazardof 40 mm Hg). The Bohr effect in SS blood ous for patients with sickling disorders.